DNA-Protein Interactions Principles and Protocols by Tom Moss

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By Tom Moss

Dr. Tom Moss assembles the hot ordinary number of state-of-the-art concepts to spot key protein-DNA interactions and outline their elements, their demeanour of interplay, and their demeanour of functionality, either within the mobilephone and within the try out tube. The recommendations span a variety, from issue id to atomic element, and contain a number of DNA footprinting analyses, together with in vivo thoughts, gel shift (EMSA) optimization, SELEX, floor plasmon resonance, site-specific DNA-protein crosslinking, and UV laser crosslinking. complete and large ranging, DNA-Protein Interactions: rules and Protocols, second variation, deals a stellar array of over a hundred up to date and with no trouble reproducible options that biochemists and molecular, mobile, and developmental biologists can use effectively this day to appreciate DNA-protein interactions.

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Kingston, R. , Moore, D. , Seidman, J. , Smith, J. ), Greene Publishing Associates/Wiley-Interscience, New York, pp. 14. 7. Walker, P. and Reeder, R. H. (1988). The Xenopus laevis ribosomal gene promoter contains a binding site for nuclear factor-1. Nucleic Acids Res. 16, 10,657–10,668. Footprinting with Exonuclease III 39 4 Footprinting with Exonuclease III Willi Metzger and Hermann Heumann 1. Introduction Within the last few years footprinting techniques have become increasingly important in the study of protein–nucleic acid interactions.

L. (1997) Transcriptional regulation of the rat poly(ADP-ribose) polymerase gene by Sp1. Eur. J. Biochem. 250, 342–353. 25. , and Schnarr, M. (1988) Specific protein–DNA complexes: immunodetection of the protein component after gel electrophoresis and Western blotting. Anal. Biochem. 174, 235–238. 26. , and Vennström, B. (1993) Identification and analysis of all components of a gel retardation assay by combination with immunoblotting. Proc. Natl. Acad. Sci. USA 90, 2574–2578. 27. Dyer, R. B. and Herzog, N.

T. and Fried, M. G. (1993) Measurement of binding kinetics using the gel electrophoresis mobility shift assay. Electrophoresis 14, 725–731. 4. Fried, M. G. and Daugherty, M. A. (1998) Electrophoretic analysis of multiple protein–DNA interactions. Electrophoresis 19, 1247–1253. 5. Cann, J. R. (1998) Theoretical studies on the mobility-shift assay of protein–DNA complexes. Electrophoresis 19, 127–141. 6. Cann, J. R. (1989) Phenomenological theory of gel electrophoresis of proteinnucleic acid complexes.

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