By Lynne S. Garcia
This 3rd version includes collaborative efforts of over a hundred and fifty skilled medical microbiologists, clinical laboratory technologists, and laboratory supervisors. This well-respected reference maintains to function the only real significant booklet offering step by step protocols and outlines that permit scientific microbiologists and laboratory employees group of workers to accomplish all analyses, together with applicable qc concepts, from the receipt of the specimen via processing, checking out, interpretation, presentation of the ultimate document, and next session. to house the increasing function of medical microbiologists, the recent version locations better emphasis on components akin to molecular methods, bioterrorism, protection, and epidemiology/infection keep watch over in scientific amenities. systems are formatted to stick to the GP02-5A (2006) record of the nationwide Committee for medical Laboratory Standards/Clinical and Laboratory criteria Institute (NCCLS/CLSI). The icons within the margin of the textual content relate to protection techniques, using general precautions, a reminder for the consumer to list correct reagent dates (receipt, opened, and expiration), in addition to to enhance qc.
the right way to Use This guide
desk of Contents
Volume 1 1. method Coding, compensation, and Billing Compliance
2. Specimen assortment, delivery, and Acceptability
three. cardio Bacteriology
four. Anaerobic Bacteriology
Volume 2 five. Antimicrobial Susceptibility trying out
6. cardio Actinomycetes
7. Mycobacteriology and Antimycobacterial Susceptibility trying out
eight. Mycology and Antifungal Susceptibility trying out
Volume three 10. Viruses and Chlamydiae
12. Molecular Diagnostics
thirteen. Epidemiologic and an infection keep an eye on Microbiology
14. caliber coverage, qc, Laboratory documents, and Water caliber
15. Biohazards and protection
Read or Download Clinical Microbiology Procedures Handbook, Volumes 1-3 PDF
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Additional resources for Clinical Microbiology Procedures Handbook, Volumes 1-3
Wet Mount for Detection of Leukocytes and Microorganisms Mary K. 3-1. 3. Processing, Isolation, Detection, and Interpretation of Aerobic Bacteriology Cultures ........................................................... 1. Paratechnical Processing of Specimens for Aerobic Bacteriology Mary K. York and Deirdre L. Church ..................... 2. Interpretation and Rapid Identification of Bacterial Growth on Primary Culture Media Mary K. York ................... 4. Blood Cultures .. 1. General Detection and Interpretation Mary K.
Allow the loop to cool thoroughly before streaking medium. A sterile plastic loop can be used in place of the sterile inoculating loop. B. Pass the cooled loop back and forth through the inoculum in the first quadrant several times. C. Do not flame the loop between the quadrants unless necessary. 1-8 Procedure for processing clinical specimens in microbiology" (continued) D. Turn the plate a quarter turn, and pass the loop through the edge of the first quadrant approximately four times while streaking into the second quadrant.
Notify physician or charge nurse that the specimens are in a fixative that kills bacteria; only molecular-probe results may be offered with this fixed specimen. Notify physician or charge nurse that a screening culture for group A streptococci only will be performed. Exceptions are transplant, cystic fibrosis, and head and neck clinic patients. Inform physician or charge nurse that specimen is mostly saliva and is not appropriate for culture. Request a repeat specimen. Notify physician or charge nurse that specimen is unsuitable for DFA.